PubMEd : hakusana"Proteus, Diabetes"
Eräiä vastauksia:
(1) Curr Pharm Biotechnol. 2019;20(7):595-604. doi: 10.2174/1389201020666190613161212.
Antibacterial
and Potential Antidiabetic Activities of Flavone C-glycosides Isolated
from Beta vulgaris Subspecies cicla L. var. Flavescens (Amaranthaceae)
Cultivated in Egypt.Eräiä vastauksia:
(1) Curr Pharm Biotechnol. 2019;20(7):595-604. doi: 10.2174/1389201020666190613161212.
Abstract
BACKGROUND:
Diabetes mellitus is the most common disease in Egypt. In this context, Beta vulgaris subspecies cicla L. var. flavescens is an edible plant that has been used in traditional medicine as a therapy for treating some diseases.
OBJECTIVES:
The current study was performed to evaluate the antibacterial and potential anti-diabetic activities of different extracts and isolated flavone C-glycoside compounds isolated from Beta vulgaris subspecies cicla L. var. flavescens leaves.
METHODS:
Phytochemical investigation of n-butanol extract led to the isolation of five phytoconstituents. Their structures were determined by spectroscopic tools, including 1D-NMR (1H- & 13C-NMR) and 2D-NMR (HMQC & HMBC) besides the comparison of the data with the literature. The extracts and phytoconstituents were evaluated in vitro for their activity against some bacterial pathogens, which represent prominent human pathogens, particularly in hospital settings. The antibacterial activity was examined against three Gram-positive bacterial strains (Staphylococcus aureus, Staphylococcus epidermidis & Enterococcus faecalis) and five Gram-negative ones (Pseudomonas aeruginosa, Proteus vulgaris, Klebsiella pneumoniae, Proteus mirabilis & Salmonella typhimurium) relative to Ciprofloxacin as a reference drug. Furthermore, the in vitro antidiabetic activity (Type II) was evaluated using the alpha-glucosidase inhibitory assay.
RESULTS:
Five flavone C-glycosides namely; Apigenin 8-C-β-D-glucopyranoside (vitexin) (1), 2''-Oxylopyranosylvitexin (2), acacetin 8-C-β-D-glucopyranoside (3), acacetin 8-C-α-L-rhamnoside (4), and 6,8-di-C-β-D-glucopyranosylapigenin (vecinin-II) (5) were isolated from n-butanol extract of B. vulgaris subspecies cicla L. var. flavescens. Compound 1 showed a promising antibacterial activity against most of the test bacterial strains with respect to the minimum inhibitory concentration values (MIC) ranged from 1.95 to 15.63 µg ml-1. On the other hand, compounds 1 and 3 demonstrated superior antidiabetic activities with IC50 values of 35.7 and 42.64 µg ml-1, respectively, while an inferior potential antidiabetic activity was recorded for compound 4 (IC50 = 145.5 µg ml-1) in comparison with Acarbose as a reference drug.
CONCLUSION:
B. vulgaris L. is an edible plant, which could be used as a natural source of antibiotic and hypoglycemic drugs.
Diabetes mellitus is the most common disease in Egypt. In this context, Beta vulgaris subspecies cicla L. var. flavescens is an edible plant that has been used in traditional medicine as a therapy for treating some diseases.
OBJECTIVES:
The current study was performed to evaluate the antibacterial and potential anti-diabetic activities of different extracts and isolated flavone C-glycoside compounds isolated from Beta vulgaris subspecies cicla L. var. flavescens leaves.
METHODS:
Phytochemical investigation of n-butanol extract led to the isolation of five phytoconstituents. Their structures were determined by spectroscopic tools, including 1D-NMR (1H- & 13C-NMR) and 2D-NMR (HMQC & HMBC) besides the comparison of the data with the literature. The extracts and phytoconstituents were evaluated in vitro for their activity against some bacterial pathogens, which represent prominent human pathogens, particularly in hospital settings. The antibacterial activity was examined against three Gram-positive bacterial strains (Staphylococcus aureus, Staphylococcus epidermidis & Enterococcus faecalis) and five Gram-negative ones (Pseudomonas aeruginosa, Proteus vulgaris, Klebsiella pneumoniae, Proteus mirabilis & Salmonella typhimurium) relative to Ciprofloxacin as a reference drug. Furthermore, the in vitro antidiabetic activity (Type II) was evaluated using the alpha-glucosidase inhibitory assay.
RESULTS:
Five flavone C-glycosides namely; Apigenin 8-C-β-D-glucopyranoside (vitexin) (1), 2''-Oxylopyranosylvitexin (2), acacetin 8-C-β-D-glucopyranoside (3), acacetin 8-C-α-L-rhamnoside (4), and 6,8-di-C-β-D-glucopyranosylapigenin (vecinin-II) (5) were isolated from n-butanol extract of B. vulgaris subspecies cicla L. var. flavescens. Compound 1 showed a promising antibacterial activity against most of the test bacterial strains with respect to the minimum inhibitory concentration values (MIC) ranged from 1.95 to 15.63 µg ml-1. On the other hand, compounds 1 and 3 demonstrated superior antidiabetic activities with IC50 values of 35.7 and 42.64 µg ml-1, respectively, while an inferior potential antidiabetic activity was recorded for compound 4 (IC50 = 145.5 µg ml-1) in comparison with Acarbose as a reference drug.
CONCLUSION:
B. vulgaris L. is an edible plant, which could be used as a natural source of antibiotic and hypoglycemic drugs.
Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.
KEYWORDS:
Amaranthaceae; Beta vulgaris subspecies cicla L. var. flavescens; antibacterial; antidiabetic; flavonoids; saponins.
(2)
Amaranthaceae; Beta vulgaris subspecies cicla L. var. flavescens; antibacterial; antidiabetic; flavonoids; saponins.
(2)
Arch Virol. 2019 Sep;164(9):2265-2275. doi: 10.1007/s00705-019-04305-x. Epub 2019 Jun 14.
Elimination of multidrug-resistant Proteus mirabilis biofilms using bacteriophages.
Abstract
Proteus mirabilis
is responsible for a wide range of infections that affect the urinary
tract, the respiratory tract, burns, wounds and the feet of individuals
with diabetes.
They are highly resistant to antimicrobial agents, and new therapeutic
options are therefore needed to combat this pathogen. The use of
bacteriophages is one option that may be useful in treating
multidrug-resistant (MDR) Proteus mirabilis infections, especially biofilm-based infections. The aim of this study was to control biofilms formed by MDR Proteus mirabilis using bacteriophages. Proteus mirabilis
isolates were identified based on biochemical tests, and their
resistance profiles were determined by the disk diffusion method. The
biofilm-forming capacity of the isolates was assessed by the
spectrophotometric method. Bacteriophages attacking Proteus mirabilis
were isolated from sewage. The effect of phage on biofilm formation was
investigated by the viable count method. A high rate of drug resistance
was found (87.2%). Strong biofilm formation was observed in 80.5% of
isolates, while moderate production was found in 19.5%. Five
bacteriophages were isolated from sewage and were tested for their
ability to eliminate biofilms. Significant disruption of pre-formed
biofilms was observed that reached up to 99.9% decrease in the number of
viable cells. The use of bacteriophages is considered a promising
strategy against the biofilm infections caused by MDR Proteus mirabilis isolates.
- PMID:
- 31197549
- DOI:
- 10.1007/s00705-019-04305-x
- [Indexed for MEDLINE]
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